I'm looking to use this as flooring for a 10x10' trade show booth. Does it need to be glued down to stay in place?

You don't have to glue our Eco-Flec Rolls to keep them in place. For use as a trade show floor, we would recommend just using some double-sided tape around the perimeter of each roll and down the seam. Then once you are finished, you can remove the tape and roll the rubber flooring so you can use it at your next trade show.