Can these Eco-Lock Rubber Tiles be used directly on vinyl plank flooring without concern of staining or should something be placed between them to protect it?

If you have LVT, WPC, or SPC, then you can install our Eco-Lock Rubber Tiles without any issues. If you have any other type of vinyl flooring, we would suggest placing a plastic sheet between the rubber flooring and vinyl. This will help prevent damage to the vinyl under the rubber.